{"id":6,"date":"2006-11-18T02:08:44","date_gmt":"2006-11-18T08:08:44","guid":{"rendered":"http:\/\/www.bigroom.org\/wordpress\/?p=6"},"modified":"2006-11-18T02:11:11","modified_gmt":"2006-11-18T08:11:11","slug":"remember-where-was-i-episode-3","status":"publish","type":"post","link":"http:\/\/www.bigroom.org\/wordpress\/?p=6","title":{"rendered":"Remember “Where Was I, Episode 3?”"},"content":{"rendered":"
\"Picture<\/center>
\nHi! My name is Willy Bacillus!<\/div>\n

“I came from the Portneuf river in Pocatello. I grew in 10% NaCl<\/span> and I look like slime-coated sausage links. I’m purple in this picture because I’ve been Gram stained<\/span>. People used to think I was just a strain of Bacillus subtilis<\/em>, but I’m really Bacillus atrophaeus<\/a><\/em>“.<\/p>\n

Yes, I named my bacterial isolates. I figured if we had to give them some arbitrary designation, naming them like hurricanes was just as good as some bizarre system with names like “ZXKUQYB276” or something equally boring.<\/p>\n

Unfortunately, we had so much trouble getting the PCR<\/span> reactions to work we ended up just pooling the class’ sequences rather than having all our own. That means I only got this one. (Actually, it looked like one of my others worked, but not well enough to give enough DNA to sequence.)<\/p>\n

I’m trying to talk the instructor into letting me do a 1-credit “Independent Study” session next semester to try to finish getting good sequence identification of Pyotr, Paul, Olga, Ursula, Peggy, Penny, Ada, Horace, and Castor.<\/p>\n

The first seven of those all produced fluorescent stuff (probably siderophores<\/span>, I’m guessing) and while I<\/em> suspect they’re probably all Pseudomonas species, and almost certainly all gamma-proteobacteria, I was told they actually could be quite different, and I’d really like to find out just how different they really are. Plus, Horace the Thermophile was just kind of neat-looking in the microscope.<\/p>\n

Yes…I am a nerd.<\/p>\n","protected":false},"excerpt":{"rendered":"

Hi! My name is Willy Bacillus! “I came from the Portneuf river in Pocatello. I grew in 10% NaCl and I look like slime-coated sausage links. I’m purple in this picture because I’ve been Gram stained. People used to think I was just a strain of Bacillus subtilis, but I’m really Bacillus atrophaeus“. Yes, I … Continue reading Remember “Where Was I, Episode 3?”<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6,5,2],"tags":[],"class_list":["post-6","post","type-post","status-publish","format-standard","hentry","category-microbiology","category-nerdity","category-where-was-i"],"_links":{"self":[{"href":"http:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/6","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6"}],"version-history":[{"count":0,"href":"http:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/6\/revisions"}],"wp:attachment":[{"href":"http:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=6"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=6"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}