{"id":131,"date":"2008-04-11T00:21:20","date_gmt":"2008-04-11T07:21:20","guid":{"rendered":"http:\/\/www.bigroom.org\/wordpress\/?p=131"},"modified":"2008-04-11T00:21:20","modified_gmt":"2008-04-11T07:21:20","slug":"fermentation-not-just-for-alcohol","status":"publish","type":"post","link":"https:\/\/www.bigroom.org\/wordpress\/?p=131","title":{"rendered":"Fermentation: not just for alcohol"},"content":{"rendered":"<p>What does gluconic acid taste like, anyway?<\/p>\n<p><a href=\"http:\/\/www.bionade.com\/bionade.php\/20_en\/20_product\/10_innovation\"><img decoding=\"async\" style=\"float:right;\" title=\"Bionade(tm) Process Diagram\" src=\"http:\/\/www.bionade.com\/img\/herstell_engl.jpg\" alt=\"\" \/><\/a>Well, that was an interesting reminder.  I&#8217;m tracking &#8220;fermentation&#8221; on <a href=\"http:\/\/www.twitter.com\">Twitter<\/a>, and caught a random reference to an interesting fermented beverage being made in Germany.  The &#8220;reminder&#8221; I drew from this serendipitous reference was that &#8220;fermentation&#8221; doesn&#8217;t necessarily mean <em>alcoholic<\/em> fermentation.<\/p>\n<p>&#8220;Fermentation&#8221; seems to be slightly tricky to define accurately.  Most definitions seem to directly mention alcohol production from sugar, but this is only an example and not a definition.  I&#8217;ve also seen the term used to mean simply &#8220;to grow a culture of microorganisms&#8221; (because the tank they are grown in can be referred to as a &#8220;fermentor&#8221;.)<\/p>\n<p>Properly speaking, fermentation is what you get when you have microbes growing under conditions where the elelectrons that get sucked away from &#8220;food&#8221; molecules like sugars ends up on another, simpler carbon compound rather than something like oxygen, and therefore fermentation is implicitly anaerobic although that&#8217;s <span class=\"moreinfo\" title=\"molecular oxygen may be involved in the reactions, but is not being used for *respiration*...\" onclick=\"alert(this.title);\">not the same as saying that fermentation cannot happen in the presence of oxygen<\/span> (e.g. the Crabtree Effect, and of course fermentation of ethanol to vinegar requires oxygen).  The end product is generally assumed to be organic acids (like acetic acid [vinegar]) or alcohols, and carbon dioxide.  So, making beer and wine is fermentation.  Making vinegar is fermentation.  Making yogurt (lactic acid) is fermentation.  Citric acid can be made by fermentation of glucose by <em>Aspergillus<\/em> molds, as can malic (apple) acid (see US Pat#3063910).  You can make tartaric (grape) acid from glucose by fermentation as well (see US Pat#2314831).<\/p>\n<p>I am familiar with the flavors of all of those products.  One I&#8217;ve never directly tasted is <a title=\"Wikipedia entry for Gluconic Acid\" target=\"_new\">gluconic acid<\/a>, which is the main product of the fermentation process used to make &#8220;<a title=\"Bionade\" href=\"http:\/\/www.bionade.com\/bionade.php\/20_en\/20_product\">BIONADE?<\/a>&#8221; (it seems to be written in all-caps everywhere).<\/p>\n<p>According to their <a title=\"BIONADE. The Innovation\">English-language page discussing their process &#8211; linked from the image at right, click to view &#8211;<\/a> they are starting with malt, just as one would for beer, but instead of <em>Saccharomyces<\/em> yeasts, they are fermenting this wort-like liquid with &#8220;acid bacteria&#8221;.  I&#8217;m going to hazard a guess that the bacterium in question is a strain of <em><a title=\"G.oxydans on NCBI Taxonomy\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/Taxonomy\/Browser\/wwwtax.cgi?id=442\">Gluconobacter oxydans<\/a><\/em> or one of its close relatives.  This group of bacteria is in the <em><a title=\"Acetobacteraceae family on NCBI Taxonomy\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/Taxonomy\/Browser\/wwwtax.cgi?id=433\">Acetobacteraceae<\/a><\/em> family of bacteria which is involved in turning your wine into vinegar.  It would appear that under the right conditions, the enzyme <a href=\"http:\/\/en.wikipedia.org\/wiki\/Glucose_oxidase\">Glucose Oxidase<\/a> (<span class=\"moreinfo\" title=\"Enzyme Commision number - each number specifies a specific enzyme-catalyzed reaction.  In this case, 1.1.3.4 refers to the conversion of glucose to D-glucono-1,5-lactone, which is what reacts spontaneously with water molecules to form gluconic acid\" onclick=\"alert(this.title);\">EC<\/span> 1.1.3.4) produced by <em>G.oxydans<\/em> converts glucose to a compound which reacts with water to form gluconic acid.  BIONADE? then adds flavor extracts and juices to the filtered fermentation product, carbonates it, and bottles it.<\/p>\n<p>Not being familiar with the flavor of gluconic acid, I&#8217;m aching to get my hands on some of this stuff and try it.<\/p>\n<p>For another example of a relatively non-alcoholic fermented beverage, see also <a title=\"Wikipedia entry for kombucha\" href=\"http:\/\/en.wikipedia.org\/wiki\/Kombucha\">Kombucha<\/a>, which is essentially sweetened tea fermented by acetic-acid bacteria and non-Saccharomyces yeasts&#8230;which I also have yet to taste.<\/p>\n<p><em>geostr:50.4600,10.2208:200804110105-06:geostr (at least if Google Maps interpretation of the address I could find at the moment is correct, and assuming the information I dug up and my interpretation of it is correct, this should be the approximate location of the brewery responsible for BIONADE? production.)<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>What does gluconic acid taste like, anyway? Well, that was an interesting reminder. I&#8217;m tracking &#8220;fermentation&#8221; on Twitter, and caught a random reference to an interesting fermented beverage being made in Germany. The &#8220;reminder&#8221; I drew from this serendipitous reference was that &#8220;fermentation&#8221; doesn&#8217;t necessarily mean alcoholic fermentation. &#8220;Fermentation&#8221; seems to be slightly tricky to &hellip; <a href=\"https:\/\/www.bigroom.org\/wordpress\/?p=131\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">Fermentation: not just for alcohol<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[48,25,9,22,15,6,5,58,8],"tags":[120,67,115,113,44,111,112,116,567,114,117,122,118,566,121,119,580,123],"class_list":["post-131","post","type-post","status-publish","format-standard","hentry","category-brew","category-computer-nerdity","category-food-science","category-freakish-eukaryotes","category-methods","category-microbiology","category-nerdity","category-want","category-why-does-it-work","tag-acetic-acid","tag-bacteria","tag-bavaria","tag-beverages","tag-brewing","tag-drink","tag-fermentation","tag-flavorings","tag-food-science","tag-germany","tag-gluconic-acid","tag-gluconobacter","tag-malic-acid","tag-microbiology","tag-non-alcoholic","tag-tartaric-acid","tag-want","tag-wild-yeasts"],"_links":{"self":[{"href":"https:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/131","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=131"}],"version-history":[{"count":0,"href":"https:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/131\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=131"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=131"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bigroom.org\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=131"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}