What shall I do?!?

Colored nigiri rice colored and shaped like 'Marshmallow Peeps'Since natural forces have not (yet) secured a replacement job for my Minister of Domestic Affairs, I’m forced to appeal to the supernatural and assume that the Spirits are angry with me for not yet having performed the appropriate Devouring Of The Soft Pink Bunnies ritual this year…so I’m devouring a package of pink “Peeps” bunnies. No doubt good fortune will finally swiftly follow…

Meanwhile, I’m poking around in patents and scientific papers, pondering a few different possible topics for the next post:

  • More on the “Nathan System” of brewing, with reference to patents and the intended purpose thereof (and pondering how to construct a homebrew-scale “Hillbilly Biotech™” version thereof)
  • Fermented foods and beverages review and request for ones I haven’t heard of yet
  • Bad poetry
  • The previously mentioned paper on getting yeast cells to eat themselves to death
  • A post composed mainly of photographs of myself with no clothes
  • The current collection of quasi-random scientific publications I’m wading through for fun
  • Some computer-related nerdity
  • Anything else

Please place your suggestions in the comments, I beg of you. Even if they are just long strings of profane threats of violence should I elect to post on [insert forbidden topic here].

“Improvements in the Fermentation and Maturation of Beers”

Judging by my webserver’s logs, almost nobody actually bothers to click through the blog-carnival host’s site to read my Giant’s Shoulders” posts. This could be due to a secret conspiracy involving famous bloggers and several shadowy government agencies. I suppose, though, that there’s a chance that simply nobody but me is that interested in non-medical microbiology. Well…today’s post is an attempt to disprove that concept, for what aspect of non-medical microbiology could be more universally appealing than beer?

Unfortunately, in the middle of trying to assemble this posting, I see the February host has decided to put the carnival up a day early, undercutting my experiment. See, I told you it was a conspiracy! I suspect the Secret Cabal of Popular Bloggers was getting pressure from the Trilateral Comission, the NSA, and Pepsico® to silence me, so they had to do it. At least being forced to miss one, I am now free from the “I’ve been posting to these since the beginning, I can’t miss one now!” treadmill.

That means, loyal readers, that you get to see this post a month before everyone else! Hooray! Stick it to The Man™! Comically paranoid rantings aside, it also means I can split this up into more than one post, which may be more readable considering how much ground the article in question actually covers. Today’s Classic Scientific Paper is:

Nathan, L:”Improvements in the fermentation and maturation of beers.”; 1930; J. Inst. Brewing; 36; pp538-550

I ran across this reference recently while working my way through an industrial microbiology text[1] that I checked out of the campus library. According to the author of this text, “The use of cylindro-conical vessels in the brewing of lager was first proposed by Nathan (1930)[…]”, referring to the now-ubiquitous style of metal fermenter seen in small brewpubs and “MegaBladderwashCo” large-scale industrial breweries alike. Based on this I had expected the reference to be a digression on the design, construction, and testing of the fermenter. When inter-library loan managed to get me a copy of the paper, I found something much more involved.

The paper is a presentation made by Dr. Leopold Nathan in 1930 to the Scottish section of the Institute of Brewing. The topic was not simply a fermenter design but the entire “Nathan System” of brewing which appears to be the basis of modern large-scale brewing, especially for Lager-type beers. At this point, Dr. Nathan had apparently already been developing this system for about thirty years (apparently starting with a German patent in 1908, which I’ve yet to find a copy of), so as you might guess it was not just a single invention but a whole collection of them. Compared to the more rustic techniques frequently in use at the time, the “Nathan System” of brewing promised to provide faster production, more consistent results, and a better final product. It does this mainly by improving the removal of “trub” (the cloudy bits of protein and such that settle out of the malt-water – the “wort” – after you boil it), preventing infection of the beer with undesirable organisms during the cooling, hops-infusion, and aeration, and by eliminating the need to “age” the brew to make it palatable. The most important improvement in the “Nathan Process” seems to be how he treats the wort between boiling and “pitching”.


For anyone unfamiliar with the brewing process, here’s a Grossly Oversimplified review of the steps:

  • Boil some malt-sugar dissolved in water to sterilize it and to help coagulate the “trub” proteins so they’ll settle out of the liquid.
  • Cool the malt solution and aerate it so that the yeast will grow in it.
  • “Pitch” your yeast into the now-cooled-and-aerated malt-water, in a container that will keep air out while letting out the carbon dioxide bubbles that the yeast will give of during the fermentation
  • Wait until the yeast get done fermenting, then put the resulting liquid into bottles/kegs/casks/whatever.

Diagram showing the containment vessel, cooling system, and sterile-air generator for the 'Nathan method' of brewing
I’ve added a couple of labels to that image from the paper, which I’m guessing was itself copied from a contemporary patent of Dr. Nathan’s. There are two purposes to this part of the Nathan Process – To cool and aerate the wort quickly without exposing it to risk of contamination, and to move trub and volatile sulfur compounds that would otherwise make the brew taste and smell funny. The hot boiled wort is pumped directly into an insulated vat (labelled “A” in the diagram) from the boiling kettle. At this stage the wort is hot enough to prevent anything from landing in it and growing. Then, the hot wort is pumped from the top of this vat into a clean-room containing a cooling device that the wort is poured on, cooling and aerating it as it flows through. Infection is prevented here by the fact that the room has a continuous stream of “sterilized” (or at least well-filtered) air, which is exhausted through the vent in the ceiling. The cooled, aerated wort is then pumped back out of the room and into the bottom of the insulated container below the still-hot wort.

Because of the large open cooling room with its constant stream of clean air, the cooling and aeration step also allows the volatile sulfurous compounds of “jungbukett” (The “Bouquet of Youth”; the unpleasant smells and tastes of immature beer, described in this paper as ‘onion-like’) to evaporate off and be carried away. Since waiting for these compounds to break down was apparently a primary reason for having to “age” lager before selling it, this not only improves the quality but eliminates the need to store the beer for months after fermentation.

The now-chilled wort then rests back in vat “A” and the trub settles out onto horizontal plates inside the vat, where it stays behind when the clarified wort is pumped out to the fermenters.

I did some poking around, and this appears to be what is described in US Patent# 1,581,194 (application filed in August of 1921), in case you are bored and want to look that up. If not, or if you don’t want to deal with the frustrating hassle of trying to view TIFF files in your browser, I intend to provide a followup post with some more details of the process and some interesting bits I found in it, and I’ll include a pdf of the patents, assuming anyone wants them.

Oh, one last thing – I’ve had no luck getting any biographical information about Dr. Leopold Nathan. Unfortunately when you search for “Leopold Nathan”, the results are clogged with references to a murdering smartass named “Nathan Leopold” instead. Doesn’t Google™ realize that brewmeisters are far more important than obscure murderers? No pictures of him, either, so I can’t even say whether his hairstyle is cooler than Eduard Buchner’s or not.

[1] Stanbury PF, Whitaker A, Hall SJ:”Principles of Fermentation Technology (2nd edition)”; 1995; Elsevier Science, Ltd; Tarrytown NY

I have a shocking confession to make.

I think I may be a nerd.

No, no, don’t try to deny it. I think it pretty much has to be true for someone who reads a 10-page scientific paper (hover or click here to see the reference) in order to learn that bacteria-snot is slimy. Yes, I am a nerd. And for that I am deeply, deeply….

Oh, who am I kidding? I like being a nerd.

Stainless Steel fermenters at a breweryFor one thing, being a nerd allows me to fully enjoy one of the perks that my job gives me – namely access to a lot of scientific papers that I otherwise wouldn’t be able to afford to obtain access to from the greedy [insert your favority profanity here] who insist on charging $30 for permission to look at a decades-old articles for a day. I should add that this perk includes Inter-Library Loan for articles that I can’t get online, and the service on campus is great so far. Same day delivery of a classic article from 1930 in what I’m guessing most people would probably consider an obscure journal.

It doesn’t have quite the same thing in it that I expected from the source that pointed me to it, but I think it can still be considered “classic”. I need to re-read it more carefully to make a final decision on this, but I think I have my next “The Giant’s Shoulders” article in time for this month’s upcoming issue. And, yes, the picture attached to this post is a hint (and, no, it’s not directly related to the bacteria-snot article mentioned above in any way…)

Since I’m sure you’re all aching to know:

According to the nutrition information on the back of the bag and some quick calculations, powdered Xanthan Gum has a density of a little over 610mg/ml (or about 10 slugs/hogshead).

I think I may be an Applied Microbiology nerd.

See, when I put dirty dishes in the sink to wash later, I often fill them up with water to soak. I didn’t see this much in Idaho, but down here in Texas I notice that if I leave them too long, the water will sometimes end up turning into a thick slime.

And here I am, wondering what kind of slime it is and if I could find a way to produce it in quantity and purify it (and then find something useful to do with it).

I’m also wondering if this delightfully simple gel electrophoresis technique might be scaled up for more production-type purposes.

This Week = No Fun, but here’s an update anyway…

Busy week with unpleasant surprises, but I ain’t dead yet. You’re probably wondering what a hot dog that’s apparently eagerly anticipating eating itself has to do with that. The answer is: nothing, but it does relate to something I have been intending to post about for a long time, but haven’t since I didn’t have access to the paper…

So, in lieu of blasting out Twitter®-style updates on my Laconica feed that nobody reads anyway (a few people no doubt see the echo of them on Twitter® itself, but I don’t know if anyone cares…), here are a couple of what-I’m-doing-now updates before I go to bed:

  • I just shot off an email to the webmaster (the only contact I could find who might have the relevant information) of the Institute of Brewing and Distilling, trying to get access to a classic paper published in the Journal of the Institute of Brewing and Distilling for the upcoming “On the Shoulders of Giants” carnival.
  • There’s no way I can afford the ~$300US that it would cost to join the group, nor even at the moment to pay the typical $30 or so that greedy paywall-imprisoned publishers charge for individual articles. However, they appear to be opening up their archives to the public, for which I think they deserve substantial praise. Still, they haven’t worked their way back to the first few decades of the 20th century yet, so I had to email and ask if there was a way to get the article in question. If not, I’ll see if there’s any way to get it through inter-library loan in time.

  • “Small Things Considered” asks “What Microbiological Discovery in 2008 Did You Find Especially Interesting?” Which brings us to the “self-eating” thing.
  • The paper on caffeine’s induction of macroautophagy (“self-eating”) in yeast (and what happens when benzoic acid is also present) finally escaped from the paywall prison in January, and I now have a copy. I at least thought it was full of interesting implications (along with some useful knowledge), so I’ll hopefully be posting about it soon.

  • I’m also a computer nerd
  • Particularly when it comes to things like Linux. I’ve been thinking of trying to do some recordings on a couple of practical subjects that interest me for “Hacker Public Radio”

  • And of course, I still need to do some Xanthomonas snot Xanthan Gum experiments.

I am an attention-whorewilling to listen to my readers’ interests, so if anyone has suggestions or comments feel free to post them…

The rightful place of science, and “The definition of an intoxicating beverage”

Advocates for the repeal of prohibition carrying 'We Want Beer' signs

Just a brief point first: President Obama in his inaugural address promised to “restore science to its rightful place.” In traditional fashion, this prompted all manner of verbosity on blogs around the web concerning just what science’s rightful place actually is. Let me just take a moment to settle this question:

Science’s rightful place is kneeling at my feet in supplication and doing my bidding! AH, HA HA HA HA HA!!!!!

But that’s not what I came here to post about. Instead, I wanted to mention a paper I finally got my hands on. You may recall that some time back there were a few stories that popped up about what was said to be a “you can’t get drunk on beer” paper published back in the 1950’s. As usual, the people doing the reporting couldn’t be bothered to actually cite the paper in question, but I figured out which one it was. The paper is this one:
Greenberg LA:”The Definition of an Intoxicating Beverage”; Q J Stud Alcohol. 1955 Jun;16(2):316-25.

I have that paper. And I am quite disappointed in it in much the same way I was with Linus Pauling’s paper proposing a triple-helix structure for DNA. I did, though, learn some interesting things from Greenberg’s paper.

It turns out that the paper actually concerns the legal definition of “intoxication” and whether or not, based on this definition, beer should be classified as an “intoxicating beverage”. Greenberg actually raises some good points…but first, some amusement:

“The average alcohol content of American beers is 3.7 per cent[…]The strongest ale is 4.2 per cent” (page 320, paragraph 4)

Unfortunately, this isn’t quite as funny as it sounds, because the part I clipped out of that quote is where he says “by weight”. It’s still kinda funny though, since that would mean, in mass-market-bladderwash terms, that “The Strongest Ale” is Heineken.


This Alcohol-by-Weight-vs-Volume issue may actually be part of why American beers have a reputation for having watery, feeble beers. It evidently used to be that places like Canada were using alcohol-by-volume, while the US was using alcohol-by-weight on their labels. An otherwise-identical beer bottled in Canada would have a higher “percent alcohol” on the label than the US-bottled version, making it seem as though the American version was weaker. So, evidently it’s not really true…things like “Coors Lite” notwithstanding. (See this recent page at Fermentarium.com for more details.)

But back to the point of the paper…evidently as Greenberg was writing this paper half a century ago, there wasn’t a clear, quantitative scientific or legal definition for “intoxication”. He points out that you can’t just define it in terms of alcohol merely having noticeable effects on the drinker, since the magnitude of the unpleasant effects at low to moderate drinking levels aren’t really much different than that for (for undesirable effects) lack of sleep, distraction [remember all those studies saying talking on the cell-phone while driving is as bad as being drunk?], hunger and so forth. For that matter, I think we can all see the problem of trying to arrest anyone who is being relaxed and amiable for “public intoxication”.

Greenberg solves this problem by looking at the average blood-alcohol concentration of people arrested for “intoxication” (0.21% back in the 1950’s, evidently), and a couple of previous studies from the 1920’s and 1930’s, and he finally settles on 0.15% Blood Alcohol Concentration as a level he’ll use as the line for “definitely intoxicated”. He then proceeds to go through the common classes of alcohol-containing beverages to determine how easy it is for someone to consume enough to reach this BAC.

In summary – For hard liquor, and average person would have to consume 8 ounces plus one ounce per hour of drinking (for example, 9 ounces consumed over the course of one hour). That’s not a difficult quantity to fit into a typical stomach, so hard liquor is obviously an “intoxicating beverage”. Fortified wines (like Port, Madiera…or Thunderbird): about 18 ounces plus 2 ounces per hour – say, a pint and a half in about 4 hours. Still pretty easy to do. An ordinary wine (like, say, a Gewürtztraminer): 36 ounces plus 4 ounces/hour – or a 7-11 “Super Big Gulp®”-sized portion in two or three hours. Getting to be a fairly substantial amount of liquid, but still plausible.

And then there’s beer. Greenberg comes up with a figure of 80 ounces + 10 ounces/hour. That’s roughly three quarts within one hour, which is quite a bit more than the approximately 2 quarts that a human stomach can hold. He goes on to describe several controlled experiments on beer consumption and the resulting blood-alcohol concentration. By pushing one group to consume a gallon and a half of beer over a period of 8 hours, they were able to get up to an average of just under “intoxicated” (by Greenberg’s definition) at 0.13%. The rate of beer ingestion required to pass the “intoxication” threshhold was more than most test subjects could even manage. Therefore (Greenberg concludes) beer should probably not actually be classified as an “intoxicating beverage”

Now for the party-poopery: Greenberg explicitly points out that this is certainly not the same as saying that beer cannot impair a drinker’s performance or judgement, so just because you haven’t consumed anything stronger than beer it doesn’t mean you should be allowed behind the wheel of a steamroller. Nor does it mean that it’d be okay for an alcoholic to drink beer (since an alcoholic is very unlikely to stop with just beer). Furthermore, these days “intoxicated” is legally about half of the level that Greenberg is using – 0.08% in most places in the US as far as I can tell. By Greenberg’s measure, you can easily get that amount of alcohol out of a pitcher of beer consumed over an hour.

So, Greenberg isn’t crazy, and beer can still make you drunk, just as we all would expect. How disappointing. Still, it’s an interesting paper and I’m glad I dug it up.

On a related note, if I can get my hands on an old paper from the Journal of the Institute of Brewing, I may have found my selection for February’s Giant’s Shoulders blog carnival. Stay tuned, more to come…

Vindicated! Margarine is correlated with stupidity!

'Happy Boy' brand margarine, with creepy-looking blank-eyed 'smiling' kid on the boxI’ve been saying for years that anyone who actually wants to eat margarine has something wrong with their head. Now, there’s PROOF:
“The study suggests that if children eat certain types of food their intelligence may be boosted or significantly lowered. It singles out margarine as having particularly strong links with lower IQ scores.”

It’s still not entirely clear whether margarine makes you stupid, or if instead being stupid predisposes you to eat margarine (or both). Either way, I continue to maintain that if you want to eat vegetable oil on your bread instead of good, wholesome, delightful butter – just get some herbed olive oil and a little balsamic vinegar [it’s not butter, but it’s darned good]. Don’t run it over powdered nickel while pumping it full of hydrogen gas to make it hard, color it yellow, and then use it as make-believe butter-like-food-product because if you do, you might as well make a Play-Doh® bread-like-loaf-product to put it on.

And before anyone asks – no, I’m not affiliated with the dairy industry…though if anyone in the dairy industry feels sympathetic to my current situation with my under-equipped home lab and wants to send me an old bacteriological microscope they just happen to have sitting around, I’d be willing to consider being a paid shill independent advocate in return…

Obscure scientific papers, Mad Science, Travel, and other randomness

First – an amazingly astute observation that I’m ashamed to have not previously noticed myself (click image to go to it’s original site and see it full-size…):
Most 'Mad Scientists' are actually just 'mad engineers'...

I’m proud to say that I think testing Mad Hypotheses is great, and will continue to try to be a Mad Scientist. And a “Dirty Old Man” someday, but that’s a whole separate issue.

Second – I am really loving the perks of my new job – namely access to the college library system. I had previously mentioned (see last couple of paragraphs) a certain article that I wanted to get my hands on:

Greenberg LA:”The Definition of an Intoxicating Beverage”;Q J Stud Alcohol. 1955 Jun;16(2):316-25

Not only does the medical library have copies of a Czechoslovakian microbiology journal, the main library had a set of this old journal, too. I have my bedtime reading for tonight…

Thirdly – Another Giant’s Shoulders carnival has come and gone. I now believe that Eduard Buchner had hit upon not only a useful truth of living systems, but also a nifty alternative “mad scientist” hairstyle. Now I need to come up with one for next month. It’s been getting me thinking, though. That blog carnival is intended for “Classic” papers. Implied is that the papers are somehow important to the development of some scientific field or other. I’d like to see a variation on the “old papers” theme focussing on random old papers (where “old” might mean a few years or decades, depending on the subject) that people have found useful or interesting. Stuff that isn’t necessarily ground-breaking and has perhaps been forgotten or lost to obscurity but still has useful things to teach us. Naturally, I’m thinking especially Microbiology (and especially Microbiology other than Medicine) and Food Science. The Carnival could be called something like “Second Chance Science” or something of the sort. Just a thought.

Fourth – speaking of “Microbiology Other Than Medicine” and Food Science, apparently The National Academies of Science want to know what scientific topics people most want to read about. As usual, “microbiology” appears to have been relegated in their breakdown to merely a subset of either medicine/diseases, “biology”, and perhaps a small subset of “energy” and “Feeding the World” (no, seriously). The survey includes space to tell them what they’re missing – I heartily encourage anyone who cares to make sure you take the survey, and mention industrial and environmental microbiology and food science as subjects they shouldn’t continue to neglect.

And, finally – next week I need to make a very-long-overdue run back up to Idaho to grab some things from the old house and make sure it’s still standing, the water’s really turned off, nothing unnecessary is running, etc. 1600 miles of driving each way. Ugh. Anybody got any good recommendations for things to listen to on the trip? Other than having a chance to finally grab some things that I am missing, maybe I’ll at least have a chance to visit New Belgium Brewing Company again, since my route goes right past it. So long as I’m not driving by on Christmas day (when I assume they’ll be closed) I may have a chance.

Über alkoholische Gärung ohne Hefezellen

In my last submission to “The Giants’ Shoulders” blog carnival, we saw how the famous surgeon Dr. Joseph Lister deftly demonstrated definitively that fermentation processes were caused by live microbes rather than some sort of mysterious soluble substance that just happened to be associated with microbes. In today’s episode, we will see how Eduard Buchner definitely demonstrated that fermentation was caused by a soluble substance that was associated with microbes, and no microbes are actually needed.

Better still…they’re both right. “Wait…what?” Read on, O Seeker of Microbiological Knowledge, and be enlightened by this month’s entry: Continue reading Über alkoholische Gärung ohne Hefezellen

Nerd Reading Spasm!

Did I mention the place I work has some amazingly spiffy perks for a nerd like me?

Last night, I was poking around pubmed looking for references to yeast and erythritol (namely, do yeast interact with it, and will they metabolize it?) I found precisely one relevant reference. From 1975. In a Czechoslavokian microbiology journal. A no-longer-existent Czechoslovakian microbiology journal. Even though it was a journal published in English, I didn’t figure I’d be able to find the article I was looking for. It did turn out that the greedy (insert long string of profanity here) anti-open-access “SpringerLink®” Netherlands organization has an electronic copy of the article…which I can get limited access to for a short time for a mere $34.00. Not going to happen, obviously.

Just in case the college had a subscription that would let me get to the article at no extra cost, I checked. No such luck. But…

…The campus medical science library just two buildings over from where I work has dead-tree editions of essentially the entire journal! Im name des Nudelmonster! Instead of paying $34.00, I got a photocopy of the article for about $0.50. Bonus: As I had hoped, the article[1] reports that erythritol is not metabolized by yeasts, although it is taken up to a small extent. That means I can add erythritol (or xylitol or sorbitol or whatever) to must or wort, and it’ll still be there when the yeast finish, leaving the resulting beverage still sweet. Hooray!

Plus, I was also able to get access to an electronic copy of a review of the uses of poly-?-glutamate[2], which I was bemoaning not having access to over on an interesting Small Things Considered post recently.

Speaking of reading, one thing I really could use are any worthwhile books on the general subject of applied/industrial microbiology, bioprocess engineering, fermentation, and so on. “Worthwhile” here means practical texts that are A)primarily about microbiological processes (as opposed to, say, bioengineering of plants) B)Reasonably technical, and C)Either “not very old” or “very old indeed” (I collect old science books).

I’m not a fan of Amazon.com’s abuses of the patent system, but I’m in a hurry since it’s past my bedtime already. Therefore, purely as a sampling of the kinds of books that sounded interesting to me, here is a selection in more or less random order of books that came up in a quick search on amazon.com. Anybody out there have any other suggestions?

Continue reading Nerd Reading Spasm!